Analysis Note

Positive ControlPC 12 cells, NIH3T3 cells, Raw 264.7 cells, HeLa cells, Human small intestine tissue

Negative Control293T cells

Application

ELISA (see coments)Immunoblotting (1-5 g/ml)Immunocytochemistry (10 g/ml)Paraffin Sections (3 g/ml)

General description

This Anti-TOMM22 Mouse mAb (4G4) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TOMM22.

Purified mouse monoclonal antibody. Recognizes the ~18 kDa TOMM22 protein.

Recognizes the ~18 kDa TOMM22 protein in PC-12, NIH3T3, Raw 264.7, and HeLa cells and human small intestine tissue.

Immunogen

Full-length, recombinant, human TOMM22 (aa 1-143), expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)